小髮夾RNA
维基百科,自由的百科全书
小髮夾RNA(Short hairpin RNA,shRNA)是一種形成急轉彎(hairpin turn)結構的RNA序列,可以經由RNA干擾(RNAi)使基因表現沉默化。shRNA可利用載體導入細胞當中,並藉由U6啟動子來確保shRNA的表現。另外,shRNA可經由切割轉變成為siRNA
[编辑] 參考文獻
- McIntyre G, Fanning G (2006). "Design and cloning strategies for constructing shRNA expression vectors". BMC Biotechnol. 6: 1. PMID 16396676.
- Harper S, Staber P, He X, Eliason S, Martins I, Mao Q, Yang L, Kotin R, Paulson H, Davidson B (2005). "RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model". Proc. Natl. Acad. Sci. U.S.A. 102 (16): 5820-5. PMID 15811941.
- Nielsen M, Pedersen F, Kjems J (2005). "Molecular strategies to inhibit HIV-1 replication". Retrovirology 2: 10. PMID 15715913.
- Paddison P, Caudy A, Bernstein E, Hannon G, Conklin D (2002). "Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells". Genes Dev. 16 (8): 948-58. PMID 11959843.
- Cao W, Hunter R, Strnatka D, McQueen C, Erickson R (2005). "DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response". J. Appl. Genet. 46 (2): 217-25. PMID 15876690.
生物化学物质种类 | 主要||
肽 | 氨基酸 | 核酸 | 糖类 | 核苷糖 | 脂類 | 萜类 | 类胡萝卜素 | 四吡咯 | 辅因子 | 类固醇 | 类黄酮 | 生物碱 | 聚酮 | 配糖类 | ||
Analogues of nucleic acids: | 核酸類型 | Analogues of nucleic acids: |
鹼基: | 嘌呤(腺嘌呤/鳥嘌呤) | 嘧啶(尿嘧啶/胸腺嘧啶/胞嘧啶) | |
---|---|---|
核苷: | 腺苷/去氧腺苷 | 鳥苷/去氧鳥苷 | 尿苷 | 胸苷 | 胞苷/去氧胞苷 | |
核苷酸: | AMP/UMP/GMP/CMP | ADP/UDP/GDP/CDP | ATP/UTP/GTP/CTP | cAMP/cGMP/cADPR | |
去氧核苷酸: | dAMP/TMP/dGMP/dCMP | dADP/TDP/dGDP/dCDP | dATP/TTP/dGTP/dCTP | |
核糖核酸: | RNA | mRNA | piRNA | tRNA | rRNA | ncRNA | gRNA | shRNA | siRNA | snRNA | miRNA | snoRNA | |
去氧核糖核酸: | DNA | mtDNA | cDNA | 質體 | 黏質體 | BAC | YAC | HAC | |
核酸相似物: | GNA | PNA | TNA | LNA | Morpholino |