Uroporphyrinogen III decarboxylase
From Wikipedia, the free encyclopedia
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Uroporphyrinogen decarboxylase
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| PDB rendering based on 1jph. | |||||||||||
| Available structures: 1jph, 1jpi, 1jpk, 1r3q, 1r3r, 1r3s, 1r3t, 1r3v, 1r3w, 1r3y, 1uro | |||||||||||
| Identifiers | |||||||||||
| Symbol(s) | UROD; PCT | ||||||||||
| External IDs | OMIM: 176100 MGI: 98916 HomoloGene: 320 | ||||||||||
| EC number | 4.1.1.37 | ||||||||||
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| RNA expression pattern | |||||||||||
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| Orthologs | |||||||||||
| Human | Mouse | ||||||||||
| Entrez | 7389 | 22275 | |||||||||
| Ensembl | ENSG00000126088 | ENSMUSG00000028684 | |||||||||
| Uniprot | P06132 | Q91VW4 | |||||||||
| Refseq | NM_000374 (mRNA) NP_000365 (protein) |
NM_009478 (mRNA) NP_033504 (protein) |
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| Location | Chr 1: 45.25 - 45.25 Mb | Chr 4: 116.49 - 116.49 Mb | |||||||||
| Pubmed search | [1] | [2] | |||||||||
Uroporphyrinogen decarboxylase, also known as UROD, is a human gene.[1]
Uroporphyrinogen III decarboxylase (UroD) is a homodimeric enzyme (EC 4.1.1.37, PDB 1URO) which catalyzes the fifth step in heme biosynthesis: the elimination of carboxyl groups from the four acetate side chains of uroporphyrinogen III to yield coproporphyrinogen III.
At low substrate concentrations the reaction is believed to follow an ordered route, with the sequential removal of CO2 from the D, A, B, and C rings, whereas at higher substrate/enzyme levels a random route seems to be operative. The enzyme functions as a dimer in solution, and both the enzymes from human and tobacco have been crystallized and solved at good resolutions.
UroD is regarded as an unusual decarboxylase, since it performs decarboxylations without the intervention of any cofactors, unlike the vast majority of decarboxylases. Its mechanism has recently been proposed to proceed through substrate protonation by an arginine residue.
Silva & Ramos (2005) performed a detailed study of competing reaction mechanisms proposed for uroporphyrinogen decarboxylase.[2]
This gene encodes the fifth enzyme of the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are known to cause familial porphyria cutanea tarda and hepatoerythropoetic porphyria.[1]
[edit] References
- ^ a b Entrez Gene: UROD uroporphyrinogen decarboxylase.
- ^ Silva PJ, Ramos MJ. Density-functional study of mechanisms for the cofactor-free decarboxylation performed by uroporphyrinogen III decarboxylase. J Phys Chem B 2005;109:18195-200. doi:10.1021/jp051792s.
[edit] Further reading
- Elder GH, Lee GB, Tovey JA (1978). "Decreased activity of hepatic uroporphyrinogen decarboxylase in sporadic porphyria cutanea tarda.". N. Engl. J. Med. 299 (6): 274–8. PMID 661926.
- de Verneuil H, Bourgeois F, de Rooij F, et al. (1992). "Characterization of a new mutation (R292G) and a deletion at the human uroporphyrinogen decarboxylase locus in two patients with hepatoerythropoietic porphyria.". Hum. Genet. 89 (5): 548–52. PMID 1634232.
- Romana M, Grandchamp B, Dubart A, et al. (1991). "Identification of a new mutation responsible for hepatoerythropoietic porphyria.". Eur. J. Clin. Invest. 21 (2): 225–9. PMID 1905636.
- Garey JR, Harrison LM, Franklin KF, et al. (1990). "Uroporphyrinogen decarboxylase: a splice site mutation causes the deletion of exon 6 in multiple families with porphyria cutanea tarda.". J. Clin. Invest. 86 (5): 1416–22. PMID 2243121.
- Garey JR, Hansen JL, Harrison LM, et al. (1989). "A point mutation in the coding region of uroporphyrinogen decarboxylase associated with familial porphyria cutanea tarda.". Blood 73 (4): 892–5. PMID 2920211.
- Roméo PH, Raich N, Dubart A, et al. (1986). "Molecular cloning and nucleotide sequence of a complete human uroporphyrinogen decarboxylase cDNA.". J. Biol. Chem. 261 (21): 9825–31. PMID 3015909.
- Dubart A, Mattei MG, Raich N, et al. (1986). "Assignment of human uroporphyrinogen decarboxylase (URO-D) to the p34 band of chromosome 1.". Hum. Genet. 73 (3): 277–9. PMID 3460962.
- Romana M, Dubart A, Beaupain D, et al. (1987). "Structure of the gene for human uroporphyrinogen decarboxylase.". Nucleic Acids Res. 15 (18): 7343–56. PMID 3658695.
- de Verneuil H, Grandchamp B, Beaumont C, et al. (1986). "Uroporphyrinogen decarboxylase structural mutant (Gly281----Glu) in a case of porphyria.". Science 234 (4777): 732–4. PMID 3775362.
- Roberts AG, Elder GH, De Salamanca RE, et al. (1995). "A mutation (G281E) of the human uroporphyrinogen decarboxylase gene causes both hepatoerythropoietic porphyria and overt familial porphyria cutanea tarda: biochemical and genetic studies on Spanish patients.". J. Invest. Dermatol. 104 (4): 500–2. PMID 7706766.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. PMID 8125298.
- Meguro K, Fujita H, Ishida N, et al. (1994). "Molecular defects of uroporphyrinogen decarboxylase in a patient with mild hepatoerythropoietic porphyria.". J. Invest. Dermatol. 102 (5): 681–5. PMID 8176248.
- Moran-Jimenez MJ, Ged C, Romana M, et al. (1996). "Uroporphyrinogen decarboxylase: complete human gene sequence and molecular study of three families with hepatoerythropoietic porphyria.". Am. J. Hum. Genet. 58 (4): 712–21. PMID 8644733.
- McManus JF, Begley CG, Sassa S, Ratnaike S (1996). "Five new mutations in the uroporphyrinogen decarboxylase gene identified in families with cutaneous porphyria.". Blood 88 (9): 3589–600. PMID 8896428.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. PMID 9373149.
- Whitby FG, Phillips JD, Kushner JP, Hill CP (1998). "Crystal structure of human uroporphyrinogen decarboxylase.". EMBO J. 17 (9): 2463–71. doi:. PMID 9564029.
- Mendez M, Sorkin L, Rossetti MV, et al. (1998). "Familial porphyria cutanea tarda: characterization of seven novel uroporphyrinogen decarboxylase mutations and frequency of common hemochromatosis alleles.". Am. J. Hum. Genet. 63 (5): 1363–75. PMID 9792863.
- Wang H, Long Q, Marty SD, et al. (1998). "A zebrafish model for hepatoerythropoietic porphyria.". Nat. Genet. 20 (3): 239–43. doi:. PMID 9806541.
- McManus JF, Begley CG, Sassa S, Ratnaike S (1999). "Three new mutations in the uroporphyrinogen decarboxylase gene in familial porphyria cutanea tarda. Mutation in brief no. 237. Online.". Hum. Mutat. 13 (5): 412. doi:. PMID 10338097.
- Christiansen L, Ged C, Hombrados I, et al. (1999). "Screening for mutations in the uroporphyrinogen decarboxylase gene using denaturing gradient gel electrophoresis. Identification and characterization of six novel mutations associated with familial PCT.". Hum. Mutat. 14 (3): 222–32. doi:. PMID 10477430.
Heme synthesis—note that some reactions occur in the cytoplasm and some in the mitochondrion (yellow)
[edit] References
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