Sodium orthovanadate
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| Sodium orthovanadate | |
|---|---|
| IUPAC name | Sodium vanadate(V) |
| Other names | sodium vanadate (V), sodium vanadium oxide |
| Identifiers | |
| CAS number | [13721-39-6] |
| RTECS number | YW1120000 |
| Properties | |
| Molecular formula | Na3VO4 |
| Molar mass | 183.91 g/mol |
| Appearance | white powder |
| Density | 2.16 g/cm³, solid |
| Solubility in water | soluble |
| Hazards | |
| Main hazards | Harmful. |
| NFPA 704 |
 1
2
0
|
| Flash point | Non-flammable |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
|
Sodium orthovanadate is the chemical compound Na3VO4 and contains the tetrahedral VO43−.[1] It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue. The VO43- ion binds irreversibly to the active sites of most protein tyrosine phosphatases.
It is often added to buffer solutions that are used in protein analysis in molecular biology. The aim is to preserve the phosphorylation of proteins of interest by inhibiting endogenous phosphatases present in cell lysate mixture. It is commonly used at a final concentration of 10 - 100mM. It is toxic when inhaled, swallowed or in contact with skin.
[edit] References
- ^ Greenwood, N. N.; Earnshaw, A. (1997). Chemistry of the Elements, 2nd Edition, Oxford:Butterworth-Heinemann. ISBN 0-7506-3365-4.
- Swarup, G. et al. Biochem. Biophys. Res. Comm. 107: 1104-1109 (1982).
