From Wikipedia, the free encyclopedia
| SgrS RNA |
 |
| Type: |
Gene; sRNA; |
| 2° structure: |
Predicted; Moxon SJ |
| Seed alignment: |
PubMed |
| Avg length: |
227 nucleotides |
|
SgrS is a 227 nucleotide small RNA that is activated by SgrR in Escherichia coli during glucose-phosphate stress. The nature of glucose-phosphate stress is not fully understood, but is correlated with intracellular accumulation of glucose-6-phosphate. SgrS helps cells recover from glucose-phosphate stress by base pairing with ptsG mRNA (encoding the glucose transporter) and causing its degradation in an RNase E dependent manner. Base pairing between SgrS and ptsG mRNA also requires Hfq, an RNA chaperone frequently required by small RNAs that affect their targets through base pairing. The inability of cells expressing sgrS to create new glucose transporters leads to less glucose uptake and reduced levels of glucose-6-phosphate. SgrS is an unusual small RNA in that it also encodes a 43 amino acid functional polypeptide, SgrT, which helps cells recover from glucose-phosphate stress by preventing glucose uptake. The activity of SgrT does not affect the levels of ptsG mRNA of PtsG protein. It has been proposed that SgrT exerts its effects through regulation of the glucose transporter, PtsG.
[edit] References
- Wadler CS, Vanderpool CK (2007). "A dual function for a bacterial small RNA: SgrS performs base pairing-dependent regulation and encodes a functional polypeptide". PNAS 104: 20454. doi:10.1073/pnas.0708102104. PMID 18042713.
- Vanderpool CK, Gottesman S (2007). "The Novel Transcription Factor SgrR Coordinates the Response to Glucose-Phosphate Stress". J. Bact. 189 (2): 2238–2248. doi:10.1128/JB.01689-06. PMID 17209026.
- Kawamoto H, Koide Y, Morita T, Aiba H (2006). "Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq". Mol. Microbiol. 61 (4): 1013–22. doi:10.1111/j.1365-2958.2006.05288.x. PMID 16859494.
- Kawamoto, H; Morita T, Shimizu A, Inada T, Aiba H (2005). "Implication of membrane localization of target mRNA in the action of a small RNA: mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli". Genes Dev 19: 328–338. doi:10.1101/gad.1270605. PMID 15650111.
- Vanderpool, CK; Gottesman S (2004). "Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system". Mol Microbiol 54: 1076–1089. doi:10.1111/j.1365-2958.2004.04348.x. PMID 15522088.
[edit] Reviews
- Vanderpool CK (2007). "Physiological consequences of small RNA- mediated regulation of glucose-phosphate stress". Curr. Opin. Micro. 10: 1–6. PMID 17383224.
[edit] External links
