Mandelonitrile lyase

From Wikipedia, the free encyclopedia

In enzymology, a mandelonitrile lyase (EC 4.1.2.10) is an enzyme that catalyzes the chemical reaction

mandelonitrile cyanide + benzaldehyde

Hence, this enzyme has one substrate, mandelonitrile, and two products, cyanide and benzaldehyde.

This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is mandelonitrile benzaldehyde-lyase (cyanide-forming). Other names in common use include hydroxynitrile lyase, (R)-oxynitrilase, oxynitrilase, D-oxynitrilase, D-alpha-hydroxynitrile lyase, and mandelonitrile benzaldehyde-lyase. This enzyme participates in cyanoamino acid metabolism. It has 2 cofactors: flavin, and Flavoprotein.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1JU2.

[edit] References

• IUBMB entry for 4.1.2.10
• BRENDA references for 4.1.2.10 (Recommended.)
• PubMed references for 4.1.2.10
• PubMed Central references for 4.1.2.10
• Google Scholar references for 4.1.2.10
• BECKER W, BENTHIN U, ESCHENHOF E, PFEIL E (1963). "[On the knowledge of cyanhydrin synthesis. II. Purification and properties of hydroxynitrilase from bitter almonds (Prunus communis Stokes)]". Biochem. Z. 337: 156–66. PMID 13970146. 
• Becker W and Pfeil E (1964). "Die Darstellung kristallisierter Oxynitrilase aus bitteren Mandeln (Prunus comm. Stks)". Naturwissenschaften 51: 193. doi:10.1007/BF00600723. 
• Gross M, Jacobs GH, Poulton JE (1982). "A rapid and sensitive spectrophotometric assay for prunasin hydrolase activity employing purified mandelonitrile lyase". Anal. Biochem. 119: 25–30. doi:10.1016/0003-2697(82)90660-1. PMID 6803611. 
• Xu LL, Singh BK, Conn EE (1986). "Purification and characterization of mandelonitrile lyase from Prunus lyonii". Arch. Biochem. Biophys. 250: 322–8. doi:10.1016/0003-9861(86)90733-2. PMID 3777939. 
• Yemm RS, Poulton JE (1986). "Isolation and characterization of multiple forms of mandelonitrile lyase from mature black cherry (Prunus serotina Ehrh.) seeds". Arch. Biochem. Biophys. 247: 440–5. doi:10.1016/0003-9861(86)90604-1. PMID 3717954. 

[edit] External links

The CAS registry number for this enzyme class is 9024-43-5.

• IUBMB entry for 4.1.2.10

• KEGG entry for 4.1.2.10

• BRENDA entry for 4.1.2.10

• NiceZyme view of 4.1.2.10

• EC2PDB: PDB structures for 4.1.2.10

• PRIAM entry for 4.1.2.10

• PUMA2 entry for 4.1.2.10

• IntEnz: Integrated Enzyme entry for 4.1.2.10

• MetaCyc entry for 4.1.2.10

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0046593: mandelonitrile lyase

• AMIGO entry for GO code 0046593: mandelonitrile lyase