From Wikipedia, the free encyclopedia
| DsrA RNA |
 |
| Type: |
Gene; sRNA; |
| 2° structure: |
Published; PubMed |
| Seed alignment: |
Bateman A |
| Avg length: |
85.8 nucleotides |
| Avg identity: |
93% |
|
DsrA RNA is a non-coding RNA that regulates both transcription, by overcoming transcriptional silencing by the nucleoid-associated H-NS protein, and translation, by promoting efficient translation of the stress sigma factor, RpoS. These two activities of DsrA can be separated by mutation: the first of three stem-loops of the 85 nucleotide RNA is necessary for RpoS translation but not for anti-H-NS action, while the second stem-loop is essential for antisilencing and less critical for RpoS translation. The third stem-loop, which behaves as a transcription terminator, can be substituted by the trp transcription terminator without loss of either DsrA function. The sequence of the first stem-loop of DsrA is complementary with the upstream leader portion of RpoS messenger RNA, suggesting that pairing of DsrA with the RpoS message might be important for translational regulation.
[edit] References
- [1] Sledjeski, D; Gottesman S (1995). "A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli". Proc Natl Acad Sci USA 92: 2003–2007. doi:10.1073/pnas.92.6.2003. PMID 7534408.
- [2] Majdalani, N; Cunning C, Sledjeski D, Elliott T, Gottesman S (1998). "DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription". Proc Natl Acad Sci USA 95: 12462–12467. doi:10.1073/pnas.95.21.12462. PMID 9770508.
- [3] Gottesman, S (2005). "Micros for microbes: non-coding regulatory RNAs in bacteria". Trends Genet 21: 399–404. doi:10.1016/j.tig.2005.05.008. PMID 15913835.
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