Kelch protein

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Kelch protein
Identifiers
Symbol	IGF1
Entrez	3479
HUGO	5464
OMIM	147440
PDB	1gof
RefSeq	NM_000618
UniProt	P01343
Other data

Kelch proteins (and Kelch-like proteins) are proteins that have repeated kelch domains. Most Kelch proteins also have a BTB (defn) domain. Each kelch domain forms the blade of a propellar structure, with the repeated kelch domains forming a Beta-propellar. The structure of this Beta-propellar is known but the function is not characterised.

Contents 1 Structure 2 Function 3 Organisms 4 Genes 4.1 Sources

[edit] Structure

The N-terminus of Kelch proteins has a BTB (broad-complex, tram-track and bric-a-brac) domain The BTB domain of kelch proteins (if present) allows the formation of homo- or heterodimers such that it mediated protein-protein interactions.

The C-terminus of Kelch proteins contains kelch repeats. Each kelch repeat motif is a 44-55 amino acid long peptide, usually occurring in clusters of 4 -7 repeats, forming a kelch domian.

Each kelch repeat forms a β-sheet secondary structure and forms a single 'blade' of the β-propellar tertiary structure that characterises Kelch domains. Traditionally, kelch domains have been found to have an affinity for actin filaments of a cell.

[edit] Function

The function of Kelch proteins is unknown. However they commonly seen to associate with actin tails

[edit] Organisms

The first Kelch protein (from which this family derives its name) was isolated from Drosophila. It has also been isolated in many other animals [INSERT], bacteria, fungi, and even virus (restricted to Poxviridae)

Poxviruses:

Cowpox: 6 (D11L, C18L, G3L, A57R)

Ectromelia: 4 (Named due to high % sequence identity to Vaccinia proteins)

– A55R (EMV150)

– C2L

– F3L

– A55RD (divergent to A55R, with low sequence identity)

Vaccinia: 3

– A55R

– C2L

– F3L

Monkeypox: 1

Variola: 0 (with partial Kelch domains, however none are functional)

Humans:

Causes a neuropathy disease

[edit] Genes

Ectromelia Virus

A55R (EVM150)

ATGAATAACA GCAGTGAATT AATTGCTGCG ATTAATGGAT TTAGAAATAG TGGACGATTT TGTGATATTA ATATAGTTAT TAATGATGAA AGGATAAATG CTCATAGACT CATCCTATCT GGAGCATCCG AATATTTTTC CATTCTATTT TCTAGTGATT TTATCGATTC TAATGAATAC GAAGTTAATC TAAGTCATTT AGATTATCAA AGTGTGAACG ATTTGATCGA TTACATTTAT GGGATACCTT TGAGCCTAAC TAACGATAGC GTGAAATATA TTCTTTCAAC TGCTGATTTT TTACAAATTG GATCTGCTAT TACGGAGTGT GAAAATTACA TACTTAAAAA TCTCTGTTCT AGAAACTGTA TCGATTTCTA CATATACGCT GATAAATATA ATAACAAGAA AATAGAAACA GCGTCGTTTA ACACAATATT ACGAAATATT TTGAGACTCA TTAACGATGA AAACTTTAAA TACTTGACAG AGGAATCAAT GATAAAAATT TTAAGCGATG ATATGTTAAA TATAAAAAAT GAGGATTTCG CACCACTAAT TCTCATTAAA TGGTTAGAGA GTACACAACA ACCATGCACC GTCGAGTTAC TTAGATGCCT CAGAATATCA TTGCTTTCCC CACAAGTTAT AAAATCACTT TATAGTCATC GACTGGTTAG TTCAATCTAC GAATGTATAA CATTCTTAAA CAATATAGCA TTCTTGGATG AATCATTTCC TAGATACAAT AGCATCGAGT TGATATCTAT CGGTATAAGT AATTCACGTG ATAAGATTTC CATAAACTGC TACAATCGTA AAAAAAATAC ATGGGAAATG ATATCTTCAC GTGGATATAG GTGTAGTTTC GCAGTGGCTG TCATGGATAA TATTATCTAT ATGATGGGTG GATACGATCA GTCCCCGTAT AGAAGTTCAA AGGTTATAGC ATACAATACA TGTACTAATT CTTGGATATA TGATATACCA GAGCTAAAAT ATCCGCGTTC TAATTGCGGA GGAGTTGTCG ATGACGAATA CATTTATTGT ATAGGTGGCA TACGCGATCA GGATTCATCG TTGATATCTG ATATTGATAG ATGGAAGCCA TCAAAACCAT ATTGGCAGAC GTATGCTAAA ATGCGAGAGC CAAAATGTGA TATGGGGGTT GCGATGTTAA ACGGATTAAT ATATGTCATA GGTGGAGTCG TTAAAGGTGG CACATGTACC GACACACTAG AGAGTTTATC AGAAGATGGA TGGATGATGC ATCGACGCCT TCCAATAAAA ATGTCCAATA TGTCGACGAT TGTTCATGCT GGAAAGATTT ATATATCTGG AGGTTACACC AATAGTAGTA TAGTTAATGA AATATCTAAT CTAGTCCTTA GCTATAATCC GATATATGAT GAATGGACCA AATTATCATC ATTAAATATT CCTAGAATTA ATCCTGCTCT ATGGTCAGTG CATAATAAAT TATATGTAGG AGGAATATCC GATGATGTTC AAACTAATAC ATCTGAAACA TACGACAAAG AAAAAGATTG TTGGACATTG GATAATGGTC ACGTGTTACC ATACAATTAT ATAATGTATA AATGCGAACC GATTAAACAT AAATATCCAT TGGAAAAAAT ACAGTACACG AATGATTTTC TAAAGTGTTT GGAGAGTTTT ATAGGTAGTT GA

C2L (EVM018)

ATGGAAAGCG TGATATTTTC TATCAATGGG GAAATTATAC AAGTGAATAA GGAAATTATT ACGGCGTCTC CGTATAATTT GTTTAAACGC ATTCAGGATC ACCATCTAAA GGATGAAGCG ATTATATTGA ATGGTATAAA CTATCACGCG TTTGAATCGC TATTAGACTA TATGCGCTGG AAGAAGATAA ACATCACCAT AAACAATGTA GAAATGATAC TAGTTGCTGC CATAATAATT GATGTTCCGC CTGTAGTAGA TCTATGTGTA AAAACTATGA TTCATAATAT TAATTCCACA AATTGTATAA GGATGTTTAA CTTTTCTAAA AGATATGGAA TTAAAAAACT ATATAATGCG TCGATGTCAG AAATAATCAA CAATATTACA GCGGTGACAT CCGATCCAGA ATTTGGAAAA TTATCAAAGG ATGAACTAAC AACTATCTTA TCCCACGAAA ACGTTAACGT AAATCATGAG GATGTTACAG CTATAATATT ATTAAAGTGG ATACATAAAA ATCCAAACGA TGTAGATATC ATCAACATTT TACATCCCAA GTTTATGACT AATGCTATGC GCAATGCTAT AGCATTGTTG GGATTAACTA TATCCAAATC TACAAAGCCT GTGACACGAA ATGGTATAAA ACATAATATA GTAGTCATTA AAAACTCTGA TTATATATCC ACAATAACCC ATTACTCTCC TAGAACAGAA TATTGGACGA TAGTCGGTAA TACAGATAGA CAATTCTATA ATGCAAATGT TTTACATAAT TGTCTATACA TTATTGGCGG CATGATTAAC AATAGACATG TTTATTCCGT ATCGCGGGTA GATCTTAAAA CGAAAAAATG GAAAACGGTC ACTAATATGT CGTCGTTAAA ATCAGAAGTT AGTACTTGTG TTAACAATGG AAAGTTATAT GTAATAGGAG GATTAGAATT TTCTATTTCA ACGGCTGTAG CAGAATATTT GAAACATGGC GCTTCGAAAT GGATAAGACT TCCAAACTTA ATTACTCCTA GATATTCAGG AGCATCGGTA TTCGTAAACG ATGATATATA TGTAATGGGT GGAGTTTATA CCACGTATGA GAAATATGTA GTATTAAATG ATGTGGAATG TTTCACTAAA AATCGTTGGA TAAAAAAGTC TCCCATGCCT AGACATCATA GTATAGTTTA TGCTGTAGAG TACGATGGTG ACATCTATGC AATTACTGGA ATTACTCATG AGACTCGTAA TTATCTATAC AAATATATAG TTAATGAAGA CAAATGGATA GAATTATACA TGTACTTTAA CCATGTAGGA AAGATGTTCG TGTGTTCTTG TGGTGATTAT ATCTTAATTA TAGCAGATGC AAAATACGAA TATTATCCAA AATCAAATAC TTGGAATTTG TTAGATACGT CAACTCGTAA TATCGAATAT TATGATATGT TTACTAAAGA TGAGACTCCA AAATGTAATG TAACTCATAA GTCACTACCA TCGTTTTTGA GCAACTGTGA AAAAAAATTC CTACAATAG

[edit] Sources