UDP-N-acetylmuramate-L-alanine ligase

From Wikipedia, the free encyclopedia

In enzymology, an UDP-N-acetylmuramate-L-alanine ligase (EC 6.3.2.8) is an enzyme that catalyzes the chemical reaction

ATP + UDP-N-acetylmuramate + L-alanine $\rightleftharpoons$ ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine

The 3 substrates of this enzyme are ATP, UDP-N-acetylmuramate, and L-alanine, whereas its 3 products are ADP, phosphate, and UDP-N-acetylmuramoyl-L-alanine.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is UDP-N-acetylmuramate:L-alanine ligase (ADP-forming). Other names in common use include MurC synthetase, UDP-N-acetylmuramoyl-L-alanine synthetase, uridine diphospho-N-acetylmuramoylalanine synthetase, UDP-N-acetylmuramoylalanine synthetase, L-alanine-adding enzyme, UDP-acetylmuramyl-L-alanine synthetase, UDPMurNAc-L-alanine synthetase, L-Ala ligase, uridine diphosphate N-acetylmuramate:L-alanine ligase, uridine 5'-diphosphate-N-acetylmuramyl-L-alanine synthetase, uridine-diphosphate-N-acetylmuramate:L-alanine ligase, UDP-MurNAc:L-alanine ligase, alanine-adding enzyme, and UDP-N-acetylmuramyl:L-alanine ligase. This enzyme participates in d-glutamine and d-glutamate metabolism and peptidoglycan biosynthesis.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1GQQ, 1GQY, 1J6U, 1P31, 1P3D, and 2F00.

[edit] References

IUBMB entry for 6.3.2.8
BRENDA references for 6.3.2.8 (Recommended.)
PubMed references for 6.3.2.8
PubMed Central references for 6.3.2.8
Google Scholar references for 6.3.2.8
Ito, E and Strominger JL (1962). "Enzymatic synthesis of the peptide in bacterial uridine nucleotides I. Enzymatic addition of L-alanine, D-glutamic acid, and L-lysine". J. Biol. Chem. 237: 2689–2695.
Nathenson SG, Strominger JL and Ito, E (1964). "Enzymatic synthesis of the peptide in bacterial uridine nucleotides IV. Purification and properties of D-glutamic acid-adding enzyme". J. Biol. Chem. 239: 1773–1776.
van Heijenoort J (2001). "Recent advances in the formation of the bacterial peptidoglycan monomer unit". Nat. Prod. Rep. 18: 503–19. doi:10.1039/a804532a. PMID 11699883.